Lineweaver–Burk Calculator
🔑 Enzymes & reaction rates. Use the Lineweaver–Burk (double reciprocal) formula to find reaction rate v, michaelis constant km, vmax, substrate [s]: pick the value you want, type the others, and the answer updates as you go.
Pick the ○ next to the value you want to find, fill in the others, and it solves as you type.
What each letter means
| Quantity | Unit |
|---|---|
| Reaction rate v | µmol/min |
| Michaelis constant Km | mM |
| Vmax | µmol/min |
| Substrate [S] | mM |
Worked example
Find the reaction rate v when Michaelis constant Km = 5 mM, Vmax = 10 µmol/min, Substrate [S] = 10 mM.
Using 1/v = KmVmax · 1/[S] + 1Vmax: Reaction rate v = 6.6667 µmol/min.
Frequently asked questions
What is the formula for Lineweaver–Burk (double reciprocal)?
1/v = KmVmax · 1/[S] + 1Vmax.
What do the letters stand for, and what units are used?
Reaction rate v in µmol/min, Michaelis constant Km in mM, Vmax in µmol/min, Substrate [S] in mM.
How do you calculate reaction rate v?
Put the values into 1/v = KmVmax · 1/[S] + 1Vmax. For example, with Michaelis constant Km = 5 mM, Vmax = 10 µmol/min, Substrate [S] = 10 mM, reaction rate v = 6.6667 µmol/min.
Can I solve for a different value?
Yes. In the calculator above, pick the circle next to any value (reaction rate v, michaelis constant km, vmax, substrate [s]) and fill in the others: it rearranges the formula for you.
